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KMID : 0384119930130020173
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 2 p.173 ~ p.180
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Determination of Trace Elements in Serum Using Indutively Coupled Plasma Atomic Emission Spectrometry
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김종완
이존기/임찬빈/권계철/구선회/박종우/한명섭/임홍빈
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Abstract
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Simple flow injection/inductively coupled plasma atomic emission spectrometry(ICP-AES) was applied to the measurements of the concentration of trace elements including copper, iron, magnesium, zinc, and strontium in human serum. The study
population was
obtained from 120 healthy men and women. Our laboratory standard solution used for the analysis of each of the trace elements and the calibration curves showed linearity. Reproducibility was main-tained in twenty successive assays of National
Institute
of Standards and Teonnology Standard reference Material 909(NIST SRM 909). The coefficient of variation was less than 4%. Accuracy was determined by the difference between the NIST SRM 909 screen results and certified values The screen results
were
within the certified ranges. The detection limits of copper, iron, magnesium, zinc, and strontium using ICP-AES were 5.8μg/L, 2.7μg/L, 48μg/L, 3.8μg/L and 0.102μg/L, respectively. The concentrations of copper, iron, magnesium, zinc, and
strontium
using ICP-AES in adult serum, were 0.93±0.20 mg/L, 1.38±0.53 mg/L, 20±1.84 mg/L, 0.99±0.16 mg/L and 0.042±0.012 mg/L, respectively. The correlation of magnesium value between ICP-AES(y) and atomic absorption spectrophotometry(x) was
y=0.84x+3.29(r=0.7967). Good linearity, reproducibility, detection limits, rapidity of analysis, and simultaneous multielement analysis capability with inicrosamples make this method particularly convenient for routine trace element measurements.
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KEYWORD
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